The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke



Hideto Nakajima1, 2, *, Ki-ichi Unoda1, 2, Takumi Ito1, Haruko Kitaoka1, Fumiharu Kimura2, Toshiaki Hanafusa2
1 Department of Internal Medicine, Seikeikai Hospital, Sakai 590-0024, Japan
2 Department of Internal Medicine I, Osaka Medical College, Takatsuki 569-8686, Japan


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© Nakajima et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Internal Medicine Seikeikai Hospital, 4-2-10 Koryonakamachi, Sakai, Osaka 590-0024 (Japan); Tel.: +81 72 251 8199; Fax: +81 72 259 3691; E-mail: in1045@poh.osaka-med.ac.jp


Abstract

Background:

Oxidative stress/free radical generation after ischemic stroke contributes to neuronal cell injury. We evaluated the utility of an oxidative stress marker, urinary 8-hydroxy-2-deoxyguanosine (8-OHdG), to demonstrate an association between the changes of 8-OHdG and outcomes after acute ischemic stroke.

Methods:

We enrolled 44 patients (26 males and 18 females) who visited our hospital due to acute ischemic stroke. Urine was collected on admission and on Days 7, and 8-OHdG was measured by ELISA. The relationships between 8-OHdG levels, stroke subtypes, and clinical outcomes based on the NIHSS and modified Rankin Scale (mRS) upon discharge was evaluated.

Results:

In the overall cohort, the mean urinary level of 8-OHdG on Day 7 was increased than that on Day 0. The 8-OHdG levels on Day 0 were not different between patients with poor and good outcomes. However, an increasing rate from Day 0 to 7 (Δ 8-OHdG) in stroke patients with a poor outcome(mRS ≥3) was significantly higher than those with a good outcome (mRS ≤2) (2.54 vs 39.44, p = 0.004).

Conclusions:

The biochemical changes related to 8-OHdG and oxidative stress may be considered a marker of ischemic brain injury and clinical outcome of ischemic stroke.

Keywords: Ischemic stroke, Oxidative stress, 8-OHdG, Clinical outcome, Radical scavenger, Edaravone.