The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke

Hideto Nakajima1, 2, *, Ki-ichi Unoda1, 2, Takumi Ito1, Haruko Kitaoka1, Fumiharu Kimura2, Toshiaki Hanafusa2
1 Department of Internal Medicine, Seikeikai Hospital, Sakai 590-0024, Japan
2 Department of Internal Medicine I, Osaka Medical College, Takatsuki 569-8686, Japan

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© Nakajima et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Internal Medicine Seikeikai Hospital, 4-2-10 Koryonakamachi, Sakai, Osaka 590-0024 (Japan); Tel.: +81 72 251 8199; Fax: +81 72 259 3691; E-mail:



Oxidative stress/free radical generation after ischemic stroke contributes to neuronal cell injury. We evaluated the utility of an oxidative stress marker, urinary 8-hydroxy-2-deoxyguanosine (8-OHdG), to demonstrate an association between the changes of 8-OHdG and outcomes after acute ischemic stroke.


We enrolled 44 patients (26 males and 18 females) who visited our hospital due to acute ischemic stroke. Urine was collected on admission and on Days 7, and 8-OHdG was measured by ELISA. The relationships between 8-OHdG levels, stroke subtypes, and clinical outcomes based on the NIHSS and modified Rankin Scale (mRS) upon discharge was evaluated.


In the overall cohort, the mean urinary level of 8-OHdG on Day 7 was increased than that on Day 0. The 8-OHdG levels on Day 0 were not different between patients with poor and good outcomes. However, an increasing rate from Day 0 to 7 (Δ 8-OHdG) in stroke patients with a poor outcome(mRS ≥3) was significantly higher than those with a good outcome (mRS ≤2) (2.54 vs 39.44, p = 0.004).


The biochemical changes related to 8-OHdG and oxidative stress may be considered a marker of ischemic brain injury and clinical outcome of ischemic stroke.

Keywords: Ischemic stroke, Oxidative stress, 8-OHdG, Clinical outcome, Radical scavenger, Edaravone.